불소양치용액이 소아 치은 섬유아세포의 세포활성에 미치는 영항에 관한 연구
EFFECTS OF FLUORIDE MOUTHRINSE ON CELL ACTIVITY OF GINGIVAL FIBROBLASTS OF CHILDREN
이동현, 이규미,
소속 상세정보
이동현 ( ) - 원광대학교 보건환경대학원 보건학교실
이규미 ( ) - 원광대학교 치과대학 소아치과학교실
KMID : 0358919970240010204
Abstract
결론
세포에 미치는 영향을 평가하기 위해 치은 섬유아세포를 배양후 불소양치용액을 1분간 적
용후, 도립현미경을 이용한 세포형태의 관찰과 [3H]-thymidine을 이용한
DNA 합성에 미치는 영향, MTT assay를 이용한 치은 섬유아세포의 활성도를 평가하여 다
음과 같은 결론을 내렸다.
1. 불화나트륨 양치용액을 가한 경우, 0.05%, 0.2% 농도에서 세포형태가 둥글어지고 세포
돌기의 부분적인 소실을 보이지만 배양시간이 지날수록 형태회복을 보였다.
2. 0.1% 불화석 양치용액을 가한 경우는 둥근 세포형태와 세포돌기의 상실을 보이고 형태
회복이 없었다.
3. 치은 섬유아세포 배양 1일, 3일째 불소양치용액의 DNA 합성이 감소되었지만 0.1% 불
화석 양치용액만이 대조군에 비해 유의하게 낮았다(p<0.05).
4. 치은 섬유아세포에 불소용액을 적용한 경우, 배양3일째 0.2% 불화나트륨과 0.1% 불화
석을 제외하고는 적용시간에 관계없이 대조군 수준의 세포활성을 보였다.
5. 0.05%와 0.02% 농도의 불화나트륨을 비교하면, 0.05% 농도가 0.02% 농도보다 높은 세
포활성 억제를 보이나 유의한 수준은 아니었다(p>0.05).
이상의 결과를 종합하여 볼 때 현재 불소양치용액으로 사용하고 있는 0.2% 불화나트륨과
0.1% 불화석을 실험실에서 직접 치은 섬유아세포에 가해질 경우 세포독성을 보이며, 0.05%,
0.02% 불화나트륨은 배양기간에 따라 세포활성의 회복경향을 보이므로 불소양치용액은 불
화나트륨이 불화석보다 유리하며, 농도는 0.02% 불화나트륨이 낮은 세포활성 억제를 보이므
로 적당할 것으로 사료된다.
#초록#
The use of fluoride is one of the most effective methods for caries prevention.
Fluoridation of public water supply has been recognized, for many years, as an
effective way to reduce dental caries. The fluoride supplement has been recommended
when the natural fluoride was unavailable or below the optimal range. However the
mechanism of caries prevention by fluoride has not yet been clarified and it is well
known that an overdose of fluoride results inacute and chronic toxicity, especially dental
fluerosis. Fluoride mouthrinsing solution is widely used in dentistry due to its
effectiveness in carrying anticariogenic action. Understanding the effects of fluoride
mouthrinsing solution on human gingival fibroblasts will provine the safety rationale for
its use during the caries preventive therapy.
The purpose of this study was to evaluate the cytotoxic effect of fluoride
mouthrinning solution on the human gingival fibroblast in vitro. The human gingival
fibroblasts were cultured from healthy gingiva on the extracted deciduous teeth of
children.
Cells were inoculated into a 24-well plate with 1×104 cells/well of
medium at 37℃, 100% humidity, 5% CO2 incubator for 24 hours. And the
cells were counted by using the hemocytometer at each designed study. Human gingival
fibroblasts were cultured in growth medium after one minute application range of
0.02%-0.2% NaF solution and 0.1% SnF2 solution. The cells used in this
study were between fifth to eighth passage number. The cell morphology was examined
by inverted microscope and cell proliferation was measured by incorporating
[3H]-thymidine into DNA. DNA synthesis by human gingival fibroblasts
was assessed by [3H] uptake assays while the cell activity was measured
by MTT assay.
Each concentrated fluoride mouthrinsing solution was estimated for its biocompatability
with fibroblasts by the tissue culture technique.
The results of this study were as follows :
1. It was observed that at 0.05%, 0.2% NaF mouthrinsing solution the cytoplasmic
proceses became globular. When 0.1% SnF2 mouthrinsing solution was
applied, the cytoplasmic process and cell morphology were disappeared.
2. DNA synthetic activity was reduced regardless of the concentration of the fluoride
mouthrinsing solution. However, the result is statistically insignificant except 0.1%
SnF2 mouth rinsing solution(p<0.05).
3. Our results indicate that 0.02%, 0.05% concentrations of NaF mouthrinsing solution
caused minimal cytotoxicity. But 0.2% NaF and 0.1% SnF2 concentration
were a significant difference between the cell activity in the experimental group and
control group(p<0.05).
The results suggest that direct exposure to fluoride solution inhibits gingival fibroblast
activity. Therefore, for the most effective use of fluoride use, lowering the concentration
of fluoride mouthrinsing is advisable because it maintains biocompatability and free ion
in the oral fluid.
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